this post was submitted on 23 May 2025
13 points (93.3% liked)

Microscopy

373 readers
42 users here now

Anything related to things that are too small to see them with the eye, and the tools used to observe them.

This space is quite general in scope - microscopes, microbiology, small component electronics, questions about buying optical components, etc.

founded 2 years ago
MODERATORS
13
Epithelial cells (mander.xyz)
submitted 1 week ago* (last edited 1 week ago) by Sal@mander.xyz to c/microscopy@mander.xyz
 

I followed the Gram Staining tutorial from this video to prepare a sample of my cheek cells: https://www.youtube.com/watch?v=lMoT-FmhS6A

For preparing the staining solutions I purchased crystal violet, ethanol, potassium iodide, iodine, and an already prepared safranin solution from laboratorium discounter.

The slight 3D effect is achieved by displacing the filter holder to block the light coming from one direction and achieve oblique illumination to cast a shadow (https://www.youtube.com/watch?v=9btIpf5mjyA).

The image is post-processed using Rawtherapee to increase the contrast.

Here is another photo without using the oblique illumination trick, also post-processed with rawtherapee:

no comments (yet)
sorted by: hot top controversial new old
there doesn't seem to be anything here