Both are cubensis, different varieties. (Taman Negara on left, B+ on right)
this post was submitted on 20 Aug 2023
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It's not that they're just different varieties, they're from different combinations of prokaryotes producing different eukaryotic mycelium. You could take the same variety, inoculate with it's spores and produce two different looking mycelium mats. It has to do with the genders (for cubensis I believe there are 12) of the spores that produce prokartotic mycelium each, and come into contact, trade neuclei and become a single eukaryotic colony. Some pairs of genders won't interact and the rest will produce different consistencies of mycelium in different combinations.
The one on the left will produce significantly more fruit bodies.
That’s so cool, thanks for commenting! While I knew the variety didn’t necessarily predict the outcome of the mycelium, I didn’t know why.
Is there a way to promote a specific mycelial mat?
Nope, it's all chance. There's a way to breed them though.
So the first thing to know is mycelium colonies ascience, which means they get old and die after a time. This means you can't just keep using the same mat and taking a culture and growing it over and over.
What you want to do is make a bunch of spore inoculations on agar in petri dishes, then take the best ones and clone them into several clones, like a dozen or two dozen, a lot. These clones are only the 1st reproduction you've done with the mat, so they're not even close to wearing out. But now you have a bunch that you can clone from. Keep them in a refrigerator, labelled.
Pick one of the clones, inoculate from it, save it. Do that until that clone is done, then move on to the next. You can basically keep a good mycelium going this way for a very long time, the limiting factor is how many initial clones you make, and that they do continue living under refrigeration, just at a much slower pace.
The approach some take is to make a bunch of spore inoculated petri dishes, then inoculate something with a culture from each one, see how it goes, then clone the shit out of the top performers into petri dishes and use cultures from those to inoculate until it runs out. Generally you can avoid a lot of wasted time by only doing this with ropy looking mycelium.
Awesome, I’ll have to give this a try. Thank you for the info!!
I’ve been wondering, does a liquid culture always result in the same mycelial mat, or is that still going to go through the same process?
It depends how the culture was started. If it was started from a clone, yes it will always result in the same mat, taking ascience into account. If it is from spores it will always go through the same process. The medium makes no difference.
I was more asking if a liquid culture would have already gone through that initial process and settled on a gender mix, meaning that specific culture would produce the same mat until it’s exhausted?
Yes, if it's a mycelium culture and not a spore syringe. If it's a spore syringe, no.